Imagine a cell mutation that allows the cell to ignore anchorage dependency. speculate on what might be the results of this mutation.

The cells need to have adhered to or to come in contact with the other layer of cells, this phenomenon is termed as anchorage dependence. The cells can get connected with the extracellular matrix, with other cells, or tissue culture plastics through proteins. In any way, various kinds of cells need some kind of anchorage in order to thrive.  

However, if a mutation permits the cell to overlook anchorage dependency, then the cells might proliferate and travel off. This generally takes place when cancer goes through metastasis and affects the entire body.

during the process of dna replication, brdu can replace thymidine and incorporate into the newly synthesized dna, cell proliferation thus can be reflected in the extent of brdu incorporation.

the solute can move "downhill," from regions of higher to lower concentration, relying on the specificity of the protein carrier to pass through the membrane. this process is called passive transport or facilitated diffusion, and does not require energy.