When making transgenic mice, to determine whether there has been stable integration of a transgene into the mouse chromosome, tail biopsies are taken from the mouse pups and tested for the presence of the transgene by

Western blot

Explanation: western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins.

i think the answer would be d

c) 50% there are four chromosomes. after crossing over, 2 of the four have been changed. that means any gamete has a 2 in 4 chance or 50% chance of receiving the mutated chromosome.