What is the most appropriate method to prepare a sample of cheek cells for pcr?

Extraction of genomic DNA

Explanation:

Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.

If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.

a fossil fuel is a fuel formed by natural processes, such as anaerobic decomposition of buried dead organisms, containing energy originating in ancient photosynthesis.[1] the age of the organisms and their resulting fossil fuels is typically millions of years, and sometimes exceeds 650 million years.[2] fossil fuels contain high percentages of carbon and include petroleum, coal, and natural gas.[3] other commonly used derivatives include kerosene and propane. fossil fuels range from volatile materials with low carbon to hydrogen ratios like methane, to liquids like petroleum, to nonvolatile materials composed of almost pure carbon, like anthracite coal. methane can be found in hydrocarbon fields either alone, associated with oil, or in the form of methane clathrates.

the theory that fossil fuels formed from the fossilized remains of dead plants by exposure to heat and pressure in the earth's crust over millions of years was first introduced by andreas libavius "in his 1597 alchemia [alchymia]" and later by mikhail lomonosov "as early as 1757 and certainly by 1763".[4] the first use of the term "fossil fuel" was by the german chemist caspar neumann, in english translation in 1759.[5]

in 2017 the world's primary energy sources consisted of petroleum (34%), coal (28%), natural gas (23%), amounting to an 85% share for fossil fuels in primary energy consumption in the world.[6] non-fossil sources in 2006 included nuclear (8.5%), hydroelectric (6.3%), and others (geothermal, solar, tidal, wind, wood, waste) amounting to 0.9%.[7] world energy consumption was growing at about 2.3% per year. in 2015 about 18% of worldwide consumption was from renewable sources.[8]