Biology
Biology, 24.12.2019 07:31, lydiapoetz2414

Aeukaryotic gene has "sticky ends" produced by the restriction endonuclease ecori. the gene is added to a mixture containing ecori and a bacterial plasmid that carries two genes conferring resistance to the antibiotics ampicillin and tetracycline. the plasmid has one recognition site for ecori located in the tetracycline resistance gene. this mixture is incubated for several hours, exposed to dna ligase, and then added to bacteria growing in nutrient broth. the bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics. bacteria containing a plasmid into which the eukaryotic gene has been inserted would grow in which of the following conditions? a) in the nutrient broth plus ampicillin, but not in the broth containing tetracycline. b) only in the broth containing both antibiotics. c) in the broth containing tetracycline, but not in the broth containing ampicillin. d) in all four types of broth. e) in the nutrient broth without antibiotics only.

answer
Answers: 2

Other questions on the subject: Biology

image
Biology, 22.06.2019 02:30, arelyhuerta
Contrast how pollination is different among gymnosperms and angiosperms.
Answers: 3
image
Biology, 22.06.2019 03:30, person76
Apair of fruit flies reproduces and has 1,000 offspring. all 1,000 of the offspring have the alleles gg. what is the most likely combination of alleles for each parent
Answers: 1
image
Biology, 22.06.2019 20:00, CJohnson9552
Which of the following is the scientific term for an organism that makes its food from inorganic materials? a. autotroph b. heterotroph c. embryotroph
Answers: 1
image
Biology, 22.06.2019 21:00, Daveandemily17
Agram of fat oxidized by cellular respiration produces approximately as much atp as a gram of carbohydrate.
Answers: 1
Do you know the correct answer?
Aeukaryotic gene has "sticky ends" produced by the restriction endonuclease ecori. the gene is added...

Questions in other subjects: