Biology
Biology, 18.12.2019 04:31, BELLE6284

The questions below will determine which restriction enzyme should be used to make a plasmid that contains both an intact vgp gene and a functional ampr gene. answer questions 1-4 by selecting from the answer choices on the left. drag the correct answer to the right of each question. part (b) inserting the vgp gene into a cloning vector in the second step of the cloning procedure, dna fragments produced by a restriction enzyme are inserted into a cloning vector that has been cut by the same enzyme. the vector used in this tutorial is plasmid. to clone the vgp gene into the plasmid below, a restriction enzyme must do all of the following: a) cut the human dna on both sides of the vgp gene, but not inside the gene b) cut the plasmid without cutting inside the ampicillin resistance gene (ampr ), shown in blue (the importance of the ampr gene will be explained in part c.) c) produce sticky ends when it cuts both the human dna and the plasmid, so that the human dna fragments can combine with the plasmid

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