In 1906 harden and young, in a series of classic studies on the fermentation of glucose to ethanol and co2 by extracts of brewer's yeast, made the following observations.(a) inorganic phosphate was essential to fermentation; when the supply of phosphate was exhausted, fermentation ceased before all the glucose was used.(b) during fermentation under these conditions, ethanol, co2, and a sugar phosphate accumulated.(c) when arsenate was substituted for phosphate, no sugar phosphate acumulated, but the fermentation proceeded until all the glucose was converted to ethanol and co2.answer the following questions.1. which enzyme of glycolysis requires inorganic phosphate and therefore stops when no phosphate is available? (a) glyceraldehyde 3-phosphate dehydrogenase(b) phosphoglycerate mutase(c) phosphofructokinase-1(d) phosphoglycerate kinase

(a) glyceraldehyde 3-phosphate dehydrogenase.

Explanation:

Glyceraldehyde 3-phosphate dehydrogenase is the enzyme which requires inorganic phosphate (Pi) essentially for it's functioning. It catalyzes the conversion of glyceraldehyde 3-phosphate into 1,3-bisphosphoglyceric acid (1,3 BPG) during glycolysis.

All other enzymes of glycolysis use ATP for phosphorylation of their substrate but this is the only enzyme which rather uses an inorganic phosphate to phosphorylate it's substrate.

the oxygenated blood then leaves the lungs through pulmonary veins, which return it to the left heart, completing the pulmonary cycle. this blood then enters the left atrium, which pumps it through the mitral valve into the left ventricle. from the left ventricle, the blood passes through the aortic valve to the aorta.

deoxygenated blood leaves through the right ventricle through the pulmonary artery. from the right atrium, the blood is pumped through the tricuspid valve (or right atrioventricular valve), into the right ventricle.