Suppose a scientist identified two yeast mutants, called huh1 and huh2. the mutants have constitutive expression of the nop operon, which includes the genes nop1 and nop2. one of the mutants is a point mutation in a dna-binding site, and the other is a deletion of the gene which encodes the dna-binding protein.

which term describes the regulatory function of the dna-binding protein?

the scientist then performs a experiment mating the two haploid yeast together to determine which mutation is in the dna-binding protein and which is in the dna-binding site. the experimental setup is listed below, wherein a minus indicates a lose-of-function mutation, and a plus indicates the wild type allele.

how will nop1 and nop2 be expressed if huh1 encodes the dna-binding protein and huh2 is a mutation in the binding site? how will nop1 and nop2 be expressed if huh2 encodes the dna-binding protein and huh1 is a mutation in the binding site?

a neutral ph of 7 and normal organism temperature is needed for a certain enzyme to be effective.  

what would happen to the enzyme if the ph and/or temperature fluctuate greatly from the norm?  

a.the enzyme’s hydrogen bonds will break.  

b.the activation energy will increase.  

c.more product will be formed.

d.the ideal temperature and ph for the enzyme function will decrease.  

cells use diffusion to get substances in and out of themselves. that diffusion will occur across the cell membrane or the outer covering of the cell. because we are talking about the surface of the cell, surface area and volume are  important factors here. as a cell increases in size, its surface area and volume increase as well.  we call that the surface area to volume ratio (sa: vol).  but the catch is that  volume increases at a greater rate than surface  area. in other words, as a cell gets bigger, the sa: vol gets smaller. so a really small cell like bacteria is mostly cell membrane (surface) with very little contents (volume). a really big cell like an egg, is mostly insides with a super thin membrane. so what ends up happening is that the cell cannot efficiently diffuse all of the stuff it needs in/out to survive. therefore cell size is limited to about 100mm. so how do we make organisms bigger than 100mm? cells divide! by dividing and becoming multicellular, organisms are able to grow!   check out the diagrams i attached to see the effect of sa: vol. i hope this ! good luck!