Turn in this sheet (with answers) along with the printout specifed below. for this exercise, you will complete on online virtusal laborator this y and answer the questions below e was developed by the howard hughes medical institute and is found a http: lmedia. hhmi. o rg/biointeractive/vlabsimmunology/i ndex. html access this exercise online and read steps in the assay to obtain a correct and final result. answer the questioens the diagnosis and background sections. perform all the 1. based on the material in this handout, and the material online, which type of elisa is being performed (direct, indirect or capture)? diagram all the components of a representative reaction well which would indicate a positive result (i. e. the nature of the interaction of the antigen, antibodies, enzymes, sabstrates, ete.) what is the target antigen for this assay (what specifically are the patient's antibodies binding to)? which disease does a positive result indicate? how could antibodies to this antigen lead to disease symptoms (consult textbook)? 2. 3. in the experiment, the plate has been previously coated with the target antigen. what is the consequence if there are "bare spots" (parts of the assay plate not bound to the target antigen or a blocking agent)?