Chromatography columns have a limited protein binding capacity during lab #4 you should not have ''overloaded'' the ni^+2 agarose column. yet you may have noticed that the w2 fraction fluoresced slightly. if you see a band in the western blot w2 lane, what does this data say about the physical protein structure of rgfp in that lane? give a possible mw for this band based upon the data (show your work)6. (4pts) if you did ''overloaded'' your ni^+2 agarose column with a gce sample that contained too much rgfp, would you expect to see a band in the w2 lane of the western blot? if so what is its mw? would you expect to see a band in the e2 lane? if so what is its mw? 7. (3pts) you develop the western blot, what mw size band in lane e3 are you expecting to observe? if you happen to see two bands - one at the expected mw and one lower band - what might you specifically conclude about the physical protein structure of the lower band?

emerald ash borer

the emerald ash borer is a green buprestid or jewel beetle native to north-eastern asia that feeds on ash species. females lay eggs in bark crevices on ash trees, and larvae feed underneath the bark of ash trees to emerge as adults in one to two years. hope this !

prophase: we can observe the chromosomes to coil up and condense. they become visible. nuclear membrane and envelope disintigrate.s

metaphase: chromosomes align at the center, the metaphase plate. spindle fibers attach to centromeres of the sister chromatids. they meet at kinetochores.

anaphase: sister chromatids are pulled towards each opposing centromere, set on different poles.

telophase: chromosomes are now clustered up only on either sides of the cell. nuclear membranes reform, and a new cell wall forms along the cell plate. this will lead to cytokinesis, the process that divides the cell membrane and forms the cell wall.

trachea, alveoli, lung capillaries, arteries

the correct answer to this question is #5