Estimation of vmax and km by inspection although graphical methods are available for accurate determination of the vmax and km of an enzyme-catalyzed reaction (see box 6–1), sometimes these quantities can be quickly estimated by inspecting values of v0 at increasing [s]. estimate the vmax and km of the enzyme-catalyzed reaction for which the following data were obtained.
[s]mvo (um/min)
2.5 x 10-628
4.0 x 10-640
1.0 x 10-570
2.0 x 10-595
4.0 x 10-5112
1.0 x 10-5128
2.0 x 10-3139
1.0 x 10-2140