Asample of cells with a total receptor concentration of 50 m was incubated with a ligand concentration of 100 m. following the incubation period, the concentration of unbound receptors was determined to be 15 m. what is the k d for the receptor-ligand interaction?

Answer : The value of for the receptor-ligand interaction is

Explanation :

The equilibrium reaction between receptor and ligand will be:

  (forward reaction)

  (backward reaction)

where,

R = receptor

L = ligand

[RL] = receptor-ligand complex

As we know that,

The expression of is:

As we are given that:

Total [R] =

Total [L] =

Free [R] =

Bound [R] =

Bound [L] = Bound [R] =

Free [L] =

Now put all the given values in above expression, we get:

Now we have to calculate the value of for the receptor-ligand interaction.

Therefore, the value of for the receptor-ligand interaction is

ans.

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thus, the correct answer includes options and f).