Arsenate (aso43-) closely resembles pi in structure and reactivity. in the reaction catalyzed by glyceraldehyde 3-phosphate dehydrogenase, arsenate can replace phosphate in attacking the energy-rich thioester intermediate. the product of this reaction, 1-arseno- 3-phosphoglycerate, is unstable. it and other acyl arsenates are rapidly and spontaneously hydrolyzed. what is the effect of arsenate on energy generation in a cell?

The effect of arsenic poisoning result in  the loss of ATP  molecules.

Explanation:

Arsenate structurally resembles the phosphate group.In the reaction catalyzed by glyceraldehyde-3-phosphate dehydrogenase,arsenate can replace the phosphate group by attacking the energy rich thioester intermediate resulting in the formation of 1-arseno-3-phosphoglycerate which is unstable and undergo hydrolysis to form 3-phosphoglycerate.

       As a result the glycolytic pathway continues but the ATP that is normally generated from 1,3-bisphosphoglycerate by substrate lebel phosphorylation is lost due to the interference of arsenate.

Arsenite is a derivative of arsenate that interacts with lipoic acid coenzyme of pyruvate dehydrogenase enzyme thus blocking the formation of acetyl CoA which ultimately leads to loss of ATP molecules.

enzyme active site and substrate specificity

enzymes bind with chemical reactants called substrates. there may be one or more substrates for each type of enzyme, depending on the particular chemical reaction. in some reactions, a single-reactant substrate is broken down into multiple products