Which organism in the alpine biome was once nearly extinct and is now on the endangered species list?

The organism in the Alpine biome that was once nearly extinct and is now on the endangered species list is the Grizzly Bear. They are omnivorous and opportunistic when it comes to food. They are now n the endangered species list due to the shortage of food.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.