Ayoung investigator would like to express and purify a newly discovered gene. she decides to clone the gene into a plasmid that contains the sequence for a hexahistidine tag. plasmids that contain a sequence encoding a hexahistidine tag will produce a recombinant protein with an additional six histidine residues attached to the n (or c ) terminus. her gene of interest will be expressed as an n ‑terminal fusion protein containing the six residue histidine tag (n−his6−protein−c) . order the steps for construction of the expression plasm

Answer and Explanation:

The steps for construction of the expression plasma

answer; the correct answer is (beta)

c. earths continental crust is thicker than its lithosphere.

because, the lithosphere is the outermost layer of the earth/ 100 km thick and is defined by its machanical properties.the lithosphere contains oceanic and continental crust that varies in age and thickness across locations and geologic time. the lithosphere is the coolest layer of the earth in terms of temperature. the term lithosphere should not be confused with the use of "geosphere" which is used to indicate all of earth systems, including the atmosphere, hydrosphere   and biosphere. the surface of the earth can be divided into the continental crust and the oceanic crust.   the continental crust is made mostly of granite while the oceanic crust is made of basalt. the average thickness of the continental crust is 25 miles and the average thickness of the oceanic crust is 5 miles.   three types of rock, igneous, sedimentary, and metamorphic, make up the surface of the earth. that's why 3 option is preferable.