Aresearch team needs an antibody that binds to a protein of interest so that protein expression can be monitored. they use epitope tagging, adding an epitope (a sequence of amino acids) to the protein through dna recombination. in this way, they do not need to develop an antibody to the protein, but can use a known, commercially available epitope-specific antibody. however, after following procedure, they are unable to localize the protein. what is a possible reason?
a. the antibody is removed during rna processing.
b. the commercial antibodies the team used were developed from rat tissues.
c. the added epitope disrupts the function of the tagged protein.
d. the antigen-binding site does not recognize the antibody.
Answers: 2
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