You have screened for several new recessive mutations in a species of wasp. wasps homozygous for apricot (aa) have pale orange eyes. (wild-type eyes are brown.) wasps homozygous for blunt (bb) have short wings. (wild-type wings are long.) you make a pure-breeding double-mutant (apricot, blunt) line and cross it with wild-type wasps. the f1 is wild-type in appearance.

The F1 will have mostly dominant phenotype, nevertheless, some recesive phenotype appears as aa - ab and bb

Explanation:

If you make a pure-breeding double-mutant (apricot, blunt) line - you will have heterozygous ab

and if you cross it with wild-type wasps - you will have for apricot: AA - Aa and for blunt BB - Bb (as A and B are dominant gen)

Then, you could have for F1 breed:

*- ab / AA: Aa - BA

*- ab / Aa: aA - aa - bA - ba

*- ab / BB: Ba - aB

*- ab / Bb: aB - ab - bB - bb

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.

prophase: we can observe the chromosomes to coil up and condense. they become visible. nuclear membrane and envelope disintigrate.s

metaphase: chromosomes align at the center, the metaphase plate. spindle fibers attach to centromeres of the sister chromatids. they meet at kinetochores.

anaphase: sister chromatids are pulled towards each opposing centromere, set on different poles.

telophase: chromosomes are now clustered up only on either sides of the cell. nuclear membranes reform, and a new cell wall forms along the cell plate. this will lead to cytokinesis, the process that divides the cell membrane and forms the cell wall.