Why must reverse transcriptase be used to create a eukaryotic expression library?
a. reverse transcriptase is only used to create prokaryotic expression libraries
b. reverse transcriptase creates cdna from mrna in prokaryotes
c. reverse transcriptase ensures the gene is in the correct orientation within the expression vector to create protein
d. reverse transcriptase creates cdna from mrna because genes in eukaryotes have large numbers of non-coding regions.
e. no other enzymes are used to create expression libraries except restriction enzymes

Option (d).

Explanation:

Genomic library may be defined as the total genome DNA of the organism. Reverse transcriptase is the enzyme used for the formation of DNA from the RNA molecule.

The eukaryotes DNA contain large number of sequences called introns that creates a problem in the construction of the eukaryote genomic library. This problem can be solved by creating the complementary DNA from the RNA molecule free from the introns.

Thus, the correct answer is option (d).

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