Biology, 12.09.2019 21:30, jebrinlayan9012
Across was performed between a yeast strain that requires methionine and lysine for growth (met— lys—) and another yeast strain, which is met+ lys+. one hundred asci were dissected, and colonies were grown from the four spores in each ascus. cells from these colonies were tested for their ability to grow on petri plates containing either minimal medium (min), min + lysine (lys), min + methionine (met), or min + lys + met. the asci could be divided into two groups based on this analysis: group 1: in 89 asci, cells from two of the four spore colonies could grow on all four kinds of media, while the other two spore colonies could grow only on min + lys + met. group 2: in 11 asci, cells from one of the four spore colonies could grow on all four kinds of petri plates. cells from a second one of the four spore colonies could grow only on min + lys plates and on min + lys + met plates. cells from a third of the four spore colonies could only grow on min + met plates and on min + lys + met. cells from the remaining colony could only grow on min + lys + met. a. what are the genotypes of each of the spores within the two types of asci? b. are the lys and met genes linked? if so, what is the map distance between them? c. if you could extend this analysis to many more asci, you would eventually find some asci with a different pattern. for these asci, describe the phenotypes of the four spores. list these phenotypes as the ability of dissected spores to form colonies on the four kinds of petri plates.
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Across was performed between a yeast strain that requires methionine and lysine for growth (met— lys...
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