For questions #10-12, refer to the following experimental design steps. note: we are not asking for all of the necessary steps, just the correct order of the steps provided. 1. lyse the cells 2. incubate with a fluorescently-labeled secondary antibody 3. incubate with a primary antibody conjugated with agarose or magnetic beads 4. run the purified protein on a gel 5. extract the protein 6. incubate with a primary antibody with no conjugation 7. use a laser to separate the material based on fluorescence 8. treat the samples with sds 9. use a laser to excite fluorophores 10. what is the correct sequence of steps for performing a co-immunoprecipitation? a. 18—642 b. 153-84 c. 1 5834 d. 18562 11. which of the steps is unique to fluorescence-activated cell sorting (facs)? a. 1 b. 8 c. 2 d. 7 12. when performing an immunostain, which of the steps would you not perform? a. 1 b. 2 c. 6 d. 9 13. the protein arc is an immediate-early gene expressed in neurons that have recently been activated, especially after a learning event. you wish to study what proteins are expressed in only those cells activated during learning using a discovery-based approach. to carry out this experiment, you first use__followed by a. facs, chip-seq b. facs, mass spectrometry c. mass spectrometry, facs d. chip-seq, facs