All of the following techniques involve hybridization between single-stranded nucleic acid molecules except:
southern blot analysis
rflp analysis
northern blot analysis
microarray analysis

RFLP analysis.

Explanation:

RFLP (Restriction fragment length polymorphism ) may be defined as a molecular technique used to determine the location of a particular gene in the DNA sequences. The individuals among the population can be easily identified by RFLP analysis.

The restriction enzyme is used for the digestion of DNA sample. The restriction fragments are then visualized by running the fragments on gel electrophoresis. The hybridization between single stranded nucleic acid is not involved in the RFLP.

Thus, the correct answer is option (1).

an active site is a region that is present on an enzyme where the reactants or the substrate molecules we can say bind and experience a chemical reaction. the substrate is basically a reactant whose concentration is changing and that is converting into a product after binding at the active site of an enzyme