Biology, 17.07.2019 20:20, mamasmontoya

Which is appropriate for initial rate method experiments to analyze an enzyme-catalyzed reaction?
both enzyme and substrate concentrations are constant.
the temperature is varied and the rate is measured.
enzyme concentration is constant and the rate is measured at different substrate concentrations.
substrate concentration is constant and the rate is measured at different concentrations of enzyme.
the substrate and enzyme concentrations are varied inversely with respect to one another and the rate is measured.

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Which is appropriate for initial rate method experiments to analyze an enzyme-catalyzed reaction?