When are the homologous pairs of chromosomes separated?

In metaphase I, the tetrads line themselves up at the metaphase plate and homologous pairs orient themselves randomly. In anaphase I, centromeres break down and homologous chromosomes separate. In telophase I, chromosomes move to opposite poles; during cytokinesis the cell separates into two haploid cells.

Explanation:

each of the 46 original chromosomes splits into two daughter chromosomes, so there are two sets of 46 daughter chromosomes that end up in each cell. human cells have 46 chromosomes. each chromosome consists of a pair of identical chromatids attached together by a structure called a centromere.