Explain why it is necessary to use the same restriction enzyme to cut the desired gene from the source and to cut the plasmid. include a drawing or diagram in your answer.

It is necessary because of the base pairing between the desired gene and plasmid

Explanation:

Restriction enzymes are enzymes that recognize the specific DNA sequence-restriction site, double-stranded cut DNA and produce ends with a single-stranded overhang. The overhangs of the plasmid can stick together with overhangs of gene of interest by complementary base pairing.  Joining of ovarhangs is done by the enzyme DNA ligase.

this protocol describes a detailed procedure for intracellular flow cytometry staining.

step 1: initiation

initiation is the beginning of transcription. it occurs when the enzyme rna polymerase binds to a region of a gene called the promoter. this signals the dna to unwind so the enzyme can ‘‘read’’ the bases in one of the dna strands. the enzyme is now ready to make a strand of mrna with a complementary sequence of bases.

step 2: elongation

elongation is the addition of nucleotides to the mrna strand. rna polymerase reads the unwound dna strand and builds the mrna molecule, using complementary base pairs. there is a brief time during this process when the newly formed rna is bound to the unwound dna. during this process, an adenine (a) in the dna binds to an uracil (u) in the rna.

step 3: termination

termination is the ending of transcription, and occurs when rna polymerase crosses a stop (termination) sequence in the gene. the mrna strand is complete, and it detaches from dna.