A50-ml crude extract of skeletal muscle contains 32 mg of protein per ml. ten μl of the extract catalyzes a reaction at a rate of 0.14 μmol product formed per minute. the extract was fractionated by ammonium sulfate precipitation, and the fraction precipitating between 20% and 40% saturation was redissolved in10 ml. this solution contains 50 mg/ml protein. ten μl of this purifi ed fraction catalyzes the reaction at a rate of 0.65 μmol/min. (a) what is the degree of purifi cation (fold purifi cation)? (b) what is the percent yield of the enzyme recovered in the purifi ed fraction?

He did not infringe upon the policy making powers that he felt the constitution gave congress. but the determination of foreign policy became preponderantly a presidential concern. when the french revolution led to a major war between france and england, washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-british. rather, he insisted upon a neutral course until the united states could grow stronger. which provides the best objective summary of this excerpt? president washington made a good choice when he decided not to accept the recommendations of his advisors and instead insisted that the united states stay neutral during the war between france and england as president, washington believed that the united states should remain neutral in foreign policy even though he had advisors who recommended that he take sides during the war between france and england. washington did not make policy decisions during his presidency because he did not want to interfere with the policy-making powers that the constitution gave congress. washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-britis