Compare the state of an enzyme active site at a low substrate concentration and at a high substrate concentration. how does this affect the rate of reaction?

Substrate concentration: Increasing substrate concentration also increases the rate of reaction to a certain point. Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.

it marks things for degradation via the proteasome. it alters there celluar location.