Which technique can be used to determine if a sample of cells expresses both isoforms? synthesize cdna from protein x mrna using primers overlapping exons 1 and 4, followed by gel electrophoresis and band visualization. separate protein x mrna by gel electrophoresis and visualize band pattern using a dna probe complementary to exon 3. perform a pcr of protein x genomic dna using primers overlapping exons 1 and 3, followed by gel electrophoresis and band visualization. perform a restriction digest of protein x genomic dna using an endonuclease that cuts in the middle of exon 2, followed by gel electrophoresis and band visualization.

Synthesize cDNA from Protein X mRNA using primers overlapping exons 1 and 4, followed by gel electrophoresis and band visualization.

Explanation:

The term isoforms refers to the ability of a cell to form different forms of a protein that will be produced through different genes, which would not be used for the same protein.

To determine whether a sample of cells expresses the two isoforms, it is necessary for the researcher to synthesize the Protein X mRNA cDNA using the primers superimposed on exons 1 and 4, followed by gel electrophoresis and band visualization.

Exons are regions of DNA that characterize some parts of the mRNA considered to be coding. Exons are used in the process mentioned above because through them it is possible to visualize the entire coding sequence of a protein. This way it is possible to know if the sample has protein isoforms.

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answer:

the water molecules are transferred from the roots to the stem or top parts of the tall tree because of important properties of water which are adhesion, cohesion and capillary action. cohesion is the property of water molecules keeps water molecules in a combined state by sharing of bonds between them at the atomic level. adhesion allows the interaction of water molecules with other substances or substrate. adhesion will cause the attachment of water molecules to the wall of the vessel (xylem) of the plant. capillary action occurs when the adhesive force to the vessel wall is stronger than the cohesive force between water molecules. capillary action mimics the transfer of water from a tube or pipe. in this way water utilizes its property to transfer water from down to top level of the plant.