You are interested in studying human ß-globin and decide to produce the protein in bacterial cells. the ß-globin gene is a relatively simple gene with a single intron. you insert the entire gene, including the gene's promoter, in a plasmid and transform the recombinant plasmid into e. coli. you are disappointed to discover that your bacterial culture does not produce the correct protein. what are some possible explanations? (select all that apply.) a) the bacterial cell is missing rna polymerase. b) the bacterial cell produces the protein but it is rapidly degraded. c) the bacterial cell cannot splice introns. d) the bacterial cell does not recognize the eukaryote promoter. b, c, and d

answer: wheres the options

explanation:

step 1: initiation

initiation is the beginning of transcription. it occurs when the enzyme rna polymerase binds to a region of a gene called the promoter. this signals the dna to unwind so the enzyme can ‘‘read’’ the bases in one of the dna strands. the enzyme is now ready to make a strand of mrna with a complementary sequence of bases.

step 2: elongation

elongation is the addition of nucleotides to the mrna strand. rna polymerase reads the unwound dna strand and builds the mrna molecule, using complementary base pairs. there is a brief time during this process when the newly formed rna is bound to the unwound dna. during this process, an adenine (a) in the dna binds to an uracil (u) in the rna.

step 3: termination

termination is the ending of transcription, and occurs when rna polymerase crosses a stop (termination) sequence in the gene. the mrna strand is complete, and it detaches from dna.

not always but somethings can be reproduced in a laboratory.