Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as b-lactamase), an enzyme present in some penicillin- resistant bacteria. the mass of this enzyme in staphylococcus aureus is 29.6 kd. the amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 1029 g of purified penicillinase was measured as a function of the concentration of penicillin. assume that the concentration of penicillin does not change appreciably during the assay.

The answer is 5.22 mM.

Explanation:

The calculation of Vmax and Km is as follows:

y =7.6225x + 1.4602

so, when x = 0

x = 1 ÷ Vmax = 1.4602.

Vmax = 1 ÷ 1.4602 = 0.68.

Thus, Vmax of the enzyme is 0.68.

The calculation of (km) is as follows:

Y = 7.6225X + 1.4602

so, when y = 0

x = 1 ÷ Km = 5.22.

5.22 mM.

Explanation:

using y=mx+c equation

y =7.6225x + 1.4602

so, when x = 0

x = 1 ÷ Vmax = 1.4602.

Vmax = 1 ÷ 1.4602 = 0.68.

Thus, Vmax  is 0.68.

Y = 7.6225X + 1.4602

so, when y = 0

x = 1 ÷ Km = 5.22.

sexual reproduction produces offspring with novel combinations of genes. species that reproduce sexually must maintain two different types of individuals, males and females, which can limit the ability to colonize new habitats as both sexes must be present.

step 1: initiation

initiation is the beginning of transcription. it occurs when the enzyme rna polymerase binds to a region of a gene called the promoter. this signals the dna to unwind so the enzyme can ‘‘read’’ the bases in one of the dna strands. the enzyme is now ready to make a strand of mrna with a complementary sequence of bases.

step 2: elongation

elongation is the addition of nucleotides to the mrna strand. rna polymerase reads the unwound dna strand and builds the mrna molecule, using complementary base pairs. there is a brief time during this process when the newly formed rna is bound to the unwound dna. during this process, an adenine (a) in the dna binds to an uracil (u) in the rna.

step 3: termination

termination is the ending of transcription, and occurs when rna polymerase crosses a stop (termination) sequence in the gene. the mrna strand is complete, and it detaches from dna.