"in order for the stain to penetrate the impervious coat of the spore, which primary stain is steamed into the cell surface? "

Answer;

-Malachite green

Explanation;

-Malachite green is also used in endospore staining, since it can directly stain endospores within bacterial cells; here a safranin counterstain is often used.

-Because of their tough protein coats made of keratin, spores are highly resistant to normal staining procedures. The primary stain in the endospore stain procedure, malachite green, is driven into the cells with heat.

lysosome is this part of the cell is surrounded by a very thick outer membrane to protect the rest of the cell from its strong enzymes.