Record your hypothesis about what will happen when biuret solution is mixed with the solutions from test tubes 1, 2, 3, and 4 here. be sure to use scientific reasoning to support your hypothesis.

Hypothesis

                  Suppose we have four test tube each test tube is filled with solution extracted from different sources. Test tube 1 contain solution extracted from sugar cane, Test tube 2 contain solution extracted from green leaves, Test tube 3 contain solution extracted from seeds, and Test tube 4 contain egg white an albumin protein.

objectives

                 To find out which solution will react with biuret solution .

Materials and method

                 To prepare biuret solution, mix potassium sodium tartarate with cupric sulfate and sodium hydroxide. A blue solution will be formed.  

Experiment

                      Pour a small amount of biuret solution in each test tube and heat them until precipitation is formed.

Results

             After analyzing the experiment, it was found that no color changed in first three test tubes except the fourth one in which the color was changed from blue to pink-purple.

Discussion

                   It was deduced from results that appearance of pink-purple color indicate the presence of peptide bonds (proteins)

Conclusion

                 Biuret test is used for the confirmation of proteins in any food sample.

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