Biology

Biology, 18.07.2019 03:30, rah45

9. using the same ecori, clone the above cut out dna fragment into the following dna5’ acaccgagaattcgctgaa3’3’ tgtggctcttaagcgactt5’

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Biology, 15.07.2019 05:30, taylenlane4213

Aplasmid vector contains a single ecori site in the polylinker (multiple cloning site). a gene of interest is flanked by two ecori sites, one at each end. you cut the vector and the dna containing your insert with ecori, and ligate the two. how many ecori site(s) is/are present in the recombinant dna molecule and how many band(s) would you see when you run your digest of recombinant dna on a gel? (assume that there is no internal ecori site in the insert dna.)

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After cutting pglo with one of these three enzymes (ecori, psti or bamhi), a researcher then removed all the dna fragment(s) that are less than 2000 bp from the restriction digest. next, she ligated the remaining linear dna fragment back together to make a plasmid (that is now missing some dna compared to the original plasmid). finally, she transformed bacteria with this plasmid, and her transformation results indicated that the transformed bacteria were no longer ampicillin-resistant. which enzyme did she cut with? explain your answer.

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9. using the same ecori, clone the above cut out dna fragment into the following dna5’ acaccgagaattc...

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