Can someone calculate the transformation efficiency? Cos' I don't know what I am doin. T^T Volume of calcium chloride: 250 μL
Volume of plasmid solution: 10 μL
Concentration of plasmid solution: 0.12
Volume of LB nutrient broth solution: 250 μL
Volume of transformation sample transferred to the agar plate: 100 μL

Manual pipetting creates a musculoskeletal load on the neck, shoulders, and upper limbs and the type of injuries that this can lead to is called repetitive stress injuries.

See diagram.we use washers to get the area and we will be integrating with respect to xouter radius is the  distance between y = e^x and y = -1    inner radius of washer is the distance between y = 1 and y = -1    the area of one cross-section of a washer is    solid lies between x = 0 and x = 2. the volume is    if you need this expanded, then    the question doesn't say to evaluate, so we're done.   

Ithink its a im not sure